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Ilhan, M. Özcan, P. Topcuoglu, D. Sertkaya, M. All-trans retinoic acid ATRA is used in the treatment of acute promyelocytic leukemia.

Results were compared to a control group 10 patients that received induction without ATRA during the same time period.

Bone marrow or peripheral blood samples were collected from all patients on d 0 and 4. The immunphenotype, myeloperoxidase MPO , reaction and the efflux uptake of rhodamine Rh were analyzed on myeloblasts in these samples.

In vivo use of all-trans retinoic acid prior to induction chemotherapy improves complete remission rate and increases rhodamine uptake in patients with de novo acute myeloid leukemia.

T1 - In vivo use of all-trans retinoic acid prior to induction chemotherapy improves complete remission rate and increases rhodamine uptake in patients with de novo acute myeloid leukemia.

Medicine - Hematology, Oncology, Transplant. Overview Fingerprint. Access Link to publication in Scopus. Link to citation list in Scopus.

Medical Oncology , 19 1 , In: Medical Oncology , Vol. Medical Oncology. Üstün, C. In: Medical Oncology. Beksac and K. Rearrangement of the chromatin structure via DNA methylation or histone modification may play a role as well.

In vivo methods of transfecting specific mouse cells utilize the same kinds of vectors as in vitro experiments, except that the vector is injected into a specific organ.

Zhou et al. Somatic cells are first transfected with pluripotent reprogramming factors temporarily Oct4 , Sox2 , Nanog , etc.

The DNA methylation inhibitor, 5-azacytidine is also known to promote phenotypic transdifferentiation of cardiac cells to skeletal myoblasts.

In prostate cancer , treatment with androgen receptor targeted therapies induces neuroendocrine transdifferentiation in a subset of patients.

The transcription factors serve as a short term trigger to an irreversible process. The transdifferentiation liver cells observed 8 months after one single injection of pdx1.

The ectopic transcription factors turn off the host repertoire of gene expression in each of the cells. However, the alternate desired repertoire is being turned on only in a subpopulation of predisposed cells.

Determining the unique set of cellular factors that is needed to be manipulated for each cell conversion is a long and costly process that involved much trial and error.

As a result, this first step of identifying the key set of cellular factors for cell conversion is the major obstacle researchers face in the field of cell reprogramming.

An international team of researchers have developed an algorithm, called Mogrify 1 , that can predict the optimal set of cellular factors required to convert one human cell type to another.

When tested, Mogrify was able to accurately predict the set of cellular factors required for previously published cell conversions correctly.

To further validate Mogrify's predictive ability, the team conducted two novel cell conversions in the laboratory using human cells, and these were successful in both attempts solely using the predictions of Mogrify.

When examining transdifferentiated cells, it is important to look for markers of the target cell type and the absence of donor cell markers which can be accomplished using green fluorescent protein or immunodetection.

It is also important to examine the cell function, epigenome , transcriptome , and proteome profiles. Cells can also be evaluated based upon their ability to integrate into the corresponding tissue in vivo [16] and functionally replace its natural counterpart.

Generally transdifferentiation that occurs in mouse cells does not translate in effectiveness or speediness in human cells. Pang et al. However, the addition of NeuroD1 was able to increase efficiency and help cells reach maturity.

The order of expression of transcription factors can direct the fate of the cell. Iwasaki et al. It has been found for induced pluripotent stem cells that when injected into mice, the immune system of the synergeic mouse rejected the teratomas forming.

Part of this may be because the immune system recognized epigenetic markers of specific sequences of the injected cells.

However, when embryonic stem cells were injected, the immune response was much lower. Whether or not this will occur within transdifferentiated cells remains to be researched.

In order to accomplish transfection , one may use integrating viral vectors such as lentiviruses or retroviruses , non-integrating vectors such as Sendai viruses or adenoviruses , microRNAs and a variety of other methods including using proteins and plasmids ; [32] one example is the non-viral delivery of transcription factor-encoding plasmids with a polymeric carrier to elicit neuronal transdifferentiation of fibroblasts.

Integrating viral vectors have the chance to cause mutations when inserted into the genome. One method of going around this is to excise the viral vector once reprogramming has occurred, an example being Cre-Lox recombination [34] Non-integrating vectors have other issues concerning efficiency of reprogramming and also the removal of the vector.

From Wikipedia, the free encyclopedia. This article may be too technical for most readers to understand.

Please help improve it to make it understandable to non-experts , without removing the technical details. February Learn how and when to remove this template message.

Bibcode : Natur. Stem Cells. Cell Differentiation.

Bone marrow or peripheral blood samples were collected from all patients on d 0 and 4. The immunphenotype, myeloperoxidase MPO , reaction and the efflux uptake of rhodamine Rh were analyzed on myeloblasts in these samples.

In vivo use of all-trans retinoic acid prior to induction chemotherapy improves complete remission rate and increases rhodamine uptake in patients with de novo acute myeloid leukemia.

T1 - In vivo use of all-trans retinoic acid prior to induction chemotherapy improves complete remission rate and increases rhodamine uptake in patients with de novo acute myeloid leukemia.

Medicine - Hematology, Oncology, Transplant. Overview Fingerprint. Access Link to publication in Scopus. Link to citation list in Scopus. Medical Oncology , 19 1 , In: Medical Oncology , Vol.

Medical Oncology. Üstün, C. In: Medical Oncology. Beksac and K. Dalva and H. Koc and N. Konuk and O. Ilhan and M.

Topcuoglu and D. Sertkaya and M. Zhou et al. Somatic cells are first transfected with pluripotent reprogramming factors temporarily Oct4 , Sox2 , Nanog , etc.

The DNA methylation inhibitor, 5-azacytidine is also known to promote phenotypic transdifferentiation of cardiac cells to skeletal myoblasts.

In prostate cancer , treatment with androgen receptor targeted therapies induces neuroendocrine transdifferentiation in a subset of patients.

The transcription factors serve as a short term trigger to an irreversible process. The transdifferentiation liver cells observed 8 months after one single injection of pdx1.

The ectopic transcription factors turn off the host repertoire of gene expression in each of the cells. However, the alternate desired repertoire is being turned on only in a subpopulation of predisposed cells.

Determining the unique set of cellular factors that is needed to be manipulated for each cell conversion is a long and costly process that involved much trial and error.

As a result, this first step of identifying the key set of cellular factors for cell conversion is the major obstacle researchers face in the field of cell reprogramming.

An international team of researchers have developed an algorithm, called Mogrify 1 , that can predict the optimal set of cellular factors required to convert one human cell type to another.

When tested, Mogrify was able to accurately predict the set of cellular factors required for previously published cell conversions correctly.

To further validate Mogrify's predictive ability, the team conducted two novel cell conversions in the laboratory using human cells, and these were successful in both attempts solely using the predictions of Mogrify.

When examining transdifferentiated cells, it is important to look for markers of the target cell type and the absence of donor cell markers which can be accomplished using green fluorescent protein or immunodetection.

It is also important to examine the cell function, epigenome , transcriptome , and proteome profiles. Cells can also be evaluated based upon their ability to integrate into the corresponding tissue in vivo [16] and functionally replace its natural counterpart.

Generally transdifferentiation that occurs in mouse cells does not translate in effectiveness or speediness in human cells.

Pang et al. However, the addition of NeuroD1 was able to increase efficiency and help cells reach maturity. The order of expression of transcription factors can direct the fate of the cell.

Iwasaki et al. It has been found for induced pluripotent stem cells that when injected into mice, the immune system of the synergeic mouse rejected the teratomas forming.

Part of this may be because the immune system recognized epigenetic markers of specific sequences of the injected cells.

However, when embryonic stem cells were injected, the immune response was much lower. Whether or not this will occur within transdifferentiated cells remains to be researched.

In order to accomplish transfection , one may use integrating viral vectors such as lentiviruses or retroviruses , non-integrating vectors such as Sendai viruses or adenoviruses , microRNAs and a variety of other methods including using proteins and plasmids ; [32] one example is the non-viral delivery of transcription factor-encoding plasmids with a polymeric carrier to elicit neuronal transdifferentiation of fibroblasts.

Integrating viral vectors have the chance to cause mutations when inserted into the genome. One method of going around this is to excise the viral vector once reprogramming has occurred, an example being Cre-Lox recombination [34] Non-integrating vectors have other issues concerning efficiency of reprogramming and also the removal of the vector.

From Wikipedia, the free encyclopedia. This article may be too technical for most readers to understand. Please help improve it to make it understandable to non-experts , without removing the technical details.

February Learn how and when to remove this template message. Bibcode : Natur. Stem Cells. Cell Differentiation.

Nature Reviews Molecular Cell Biology. Cell Metabolism.

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Trans En Vivo - Access options

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